You are designing an experiment to investigate the presence of promoter binding sites for holoenzymes bearing B and E. in B. subtilis. A DNA template is available along with the two holoenzyme complexes and the in vitro transcription buffers. Which of the following is a suitable negative control for this experiment?
a reaction containing a DNA lacking the two promoter regions
a reaction containing all the components minus UTP
a reaction containing DNA lacking the -10 region
a reaction containing DNA lacking the -35 region
both a reaction containing a DNA lacking the two promoter regions and a reaction containing all the components minus UTP are correct